Friday, January 24, 2020
Paralysis in Dubliners Essay -- Literacy Analysis
In his letters, Joyce himself has said that Dubliners was meant ââ¬Å"to betray the soul of that hemiplegia or paralysis which many consider a cityâ⬠(55). The paralysis he was talking about is the paralysis of action. The characters in Dubliners exemplify paralysis of action in their inability to escape their lives. In another of Joyceââ¬â¢s writings, A Portrait of the Artist as a Young Man, Joyce writes of Ireland: ââ¬Å"When the soul of a man is born in this country there are nets flung at it to hold it back from flight. You talk to me of nationality, language, religion. I shall try to fly by those netsâ⬠(Joyce 238). The characters of Dubliners face similar nets that prevent them from escaping their lives. Unfortunately, their attempts to ââ¬Å"fly by those netsâ⬠are not always present; the characters often do not try to break out of their lives (as in ââ¬Å"Clayâ⬠). In the case that they do attempt to break their paralysis, the characters typically fail, or at least such is the case for the time before and during the story that the reader observes. The causes of this persistent paralysis vary between the characters: some are paralyzed by lack of motivation or fear, others by familial or other bonds; by religion, addictions, by simple lack of resources, or misconceptions or misinterpretations of events or words. Most all of the characters in the stories covered in class share fear or lack of will as the cause for their being paralyzed in their lives. To give an example, Chandler from ââ¬Å"A Little Cloudâ⬠wants to break free of his dull life to become a writer and explore the world. However, he lacks confidence in himself. ââ¬Å"Shyness had always held him back,â⬠in all aspects of his life, from reading poetry to his wife to enter... ...ple no matter what city they are in. But as Joyce shows us, the possibility is there to break out of the mold; to create a meaningful life from a once dull existence. Works Cited Brendle, Mark. "Paralysis and Epiphany in Dubliners." Web log post. Unabashedly Bookish. Barnes & Noble, 14 Oct. 2010. Web. 22 Apr. 2012. Fairhall, James. ââ¬Å"Joyce's DUBLINERS.â⬠Explicator 43.2 (1985): 28. Academic Search Premier. Web. 22 Apr. 2012. Joyce, James. A Portrait of the Artist as a Young Man. New York, B.W. Huebsch, 1922. Print. Joyce, James. Dubliners. Ed. Margot Norris. New York: W.W. Norton, 2006. Print. Joyce, James. Letters of James Joyce. Ed. Stuart Gilbert. New York: Viking, 1957. Print. Rice, Thomas Jackson. "Paradigm Lost: `Grace' And The Arrangement Of Dubliners." Studies In Short Fiction 32.3 (1995): 405. Academic Search Premier. Web. 22 Apr. 2012. Paralysis in Dubliners Essay -- Literacy Analysis In his letters, Joyce himself has said that Dubliners was meant ââ¬Å"to betray the soul of that hemiplegia or paralysis which many consider a cityâ⬠(55). The paralysis he was talking about is the paralysis of action. The characters in Dubliners exemplify paralysis of action in their inability to escape their lives. In another of Joyceââ¬â¢s writings, A Portrait of the Artist as a Young Man, Joyce writes of Ireland: ââ¬Å"When the soul of a man is born in this country there are nets flung at it to hold it back from flight. You talk to me of nationality, language, religion. I shall try to fly by those netsâ⬠(Joyce 238). The characters of Dubliners face similar nets that prevent them from escaping their lives. Unfortunately, their attempts to ââ¬Å"fly by those netsâ⬠are not always present; the characters often do not try to break out of their lives (as in ââ¬Å"Clayâ⬠). In the case that they do attempt to break their paralysis, the characters typically fail, or at least such is the case for the time before and during the story that the reader observes. The causes of this persistent paralysis vary between the characters: some are paralyzed by lack of motivation or fear, others by familial or other bonds; by religion, addictions, by simple lack of resources, or misconceptions or misinterpretations of events or words. Most all of the characters in the stories covered in class share fear or lack of will as the cause for their being paralyzed in their lives. To give an example, Chandler from ââ¬Å"A Little Cloudâ⬠wants to break free of his dull life to become a writer and explore the world. However, he lacks confidence in himself. ââ¬Å"Shyness had always held him back,â⬠in all aspects of his life, from reading poetry to his wife to enter... ...ple no matter what city they are in. But as Joyce shows us, the possibility is there to break out of the mold; to create a meaningful life from a once dull existence. Works Cited Brendle, Mark. "Paralysis and Epiphany in Dubliners." Web log post. Unabashedly Bookish. Barnes & Noble, 14 Oct. 2010. Web. 22 Apr. 2012. Fairhall, James. ââ¬Å"Joyce's DUBLINERS.â⬠Explicator 43.2 (1985): 28. Academic Search Premier. Web. 22 Apr. 2012. Joyce, James. A Portrait of the Artist as a Young Man. New York, B.W. Huebsch, 1922. Print. Joyce, James. Dubliners. Ed. Margot Norris. New York: W.W. Norton, 2006. Print. Joyce, James. Letters of James Joyce. Ed. Stuart Gilbert. New York: Viking, 1957. Print. Rice, Thomas Jackson. "Paradigm Lost: `Grace' And The Arrangement Of Dubliners." Studies In Short Fiction 32.3 (1995): 405. Academic Search Premier. Web. 22 Apr. 2012.
Thursday, January 16, 2020
Changing Hr Practices in Cable Co
Changing HR practices in Cable Co Case study Table of Content 1. Introductionâ⬠¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦.. 3 2. Problem diagnosisâ⬠¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦. 3 3. Data collection and analysisâ⬠¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦. 5 4. 1 Problem #1 ââ¬â Human Resources planningâ⬠¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦.. 5 4. 2 Problem #2 ââ¬â Performance Management Systemâ⬠¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦.. 6 4. 3 Problem #3 ââ¬â Recruitment and Selection processesâ⬠¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦ 7 4. Proposed Solutionâ⬠¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦7 5. 4 Human Resources planningâ⬠¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦8 5. 5 Performance Appraisal Systemâ⬠¦. â⬠¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦. 8 5. 6 Recruitment and Selection processâ⬠¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦10 References Listâ⬠¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦Ã¢â¬ ¦12 Appendix 1 ââ¬â The 4 stage Leadership Model Appendix 2 ââ¬â Performance Appraisal form Appendix 3 ââ¬â Giving Feedback Tool 1. Introduction This case study is based on Ca bleCo which is a joint venture between an Australian corporation AuzCo, and two Chinese organizations Post Bureau and PTA. The purpose of this report is to help the company to diagnose the problem, after research of the companyââ¬â¢s background information, identify its causes and offer a solution.This new Australia and China joint venture is located in an Economics and Development Zone about 47 km from the downtown area of Tianjing, a well-developed industrial city in China. The main products of the joint venture are telecommunications and various other kinds of communication cables. The Company has 184 employees in total; most of them 60% (113) are employed in the Production Department, as it is the most important functional part of CableCo. The Companyââ¬â¢s mission is expressed ââ¬Å"to produce quality products, provide first-class service to customers and guarantee reliable delivery. The Companyââ¬â¢s business strategy is to continuously develop new products, improve the quality of goods, reduce costs, and manage its human resources efficiently and strengthen management control. Implementation of the companyââ¬â¢s strategy depended strongly on effective employee performance and management control (Zhu, Case Study A). The telecommunication sector in China where CableCo operates has undergone rapid development. There has been a steady growth in the total number of phones in China.Because of this reason, the cable industry in China has attracted many domestic and international investors. As supply exceeds demand in this market segment, it is more difficult maintain key employees. The CableCo management team has realized that improvements in efficiency and effectiveness had to be achieved through better utilization of its human resources (Zhu, Case Study A). 2. Problem Diagnosis After the initial meeting with the client it is found that the companyââ¬â¢s authenticity is rather low in regards to managing their human resources.Although Cable Co realizes that its employees are the most valuable asset they have, the company still has a lot defects and inefficient processes in the HR management area. The key problem that the company is concerned about is how to improve effectiveness and efficiency of the Human Resources management that is the most important area in a highly competitive market. Cable Co is in danger to lose their image as a good quality product manufacturer because of uncertain business development strategy and employee internal dissatisfaction within a growing competitive market.The Management consultantââ¬â¢s main objective would be to assist the company in changing its HR practices and processes in order to improve Cable Coââ¬â¢s Human Resources Management and to enable effective operation in the highly competitive market within a multicultural working environment. A Structured model (Issue tree) enables us to see Cable Coââ¬â¢s situation more clearly. Using this model it would also facilitate in r ecognizing main causes and find the best ways for possible solutions for companyââ¬â¢s problems (Fombrun and Nevins, 2004). Inefficient Human Resources Management Problem #2Defects in performance management system Job analysis/Performance appraisals Bonus system Problem #3 Recruitment & Selection Candidate database Selection Methods Problem #1 Organizationââ¬â¢s Structure & HR Planning Short-term oriented HR planning To keep a stable workforce 3. Data collection and analysis In order to analyze the situation within Cable Co, data collection had been processed. There were two basic data collection approaches used ââ¬â deductive and naturalistic. To gain a more detailed overview of the companyââ¬â¢s situation the 4 stage leadership model is used.Structured interviews with each leadership team member were conducted. Also questionnaires based on employeesââ¬â¢ current job satisfaction, understanding of job requirements and a rewards system were used in the data collectio n process. The work environment observations and interactions between managers, supervisors and employees along with the organizationââ¬â¢s documents were an important information source during analyzing the situation in Cables Co. Based on previously constructed issue tree model, the main problem and its causes can be stated: 3. Problem #1 ââ¬â Human Resources Planning * Organizationââ¬â¢s Structure A lot of Chinese companies are operating under strict control of the government. There are unclear and different views between Chinese and Australian managers at Cable Co. Common ground rules and guidelines should be introduced. Currently some leading positions and responsibilies are overlapping or two positions are held by the same person. * Short-term oriented HR planning Both management parties (Chinese on one side, Australian on the other side) have different approaches to business development.To evaluate the demand for new employees the company uses its forecasted product ion needs, based on the market situation. Also data from AuzCo Australian are used as a standard to formulate the staffing needs in the production department. It seems that staffing needs analysis is more affected from how it is done in the West than in China, although it should be based more on Chinaââ¬â¢s market situation. Obviously judgmental forecasting is used to forecast the companyââ¬â¢s future employment needs this means that managers and other people within the company decide on staffing requirements. Gully, M. , Phillips M. 2009) There is no research data of Cable Coââ¬â¢s target market in China, and this causes management uncertainty and different opinions where the company is going and in which direction they are supposed be heading? Without knowing the companyââ¬â¢s goals, it is very difficult for the managers to agree on a common staffing strategy. * Unstable workforce High turnover in locally recruited employees make it difficult to keep workforce stable w hich affects the effectiveness of the company which reflects badly on HR recruitment.Intensifying competition in employment market, gives a lot of opportunities for employees to seek employment elsewhere. This would have an impact on Cable Coââ¬â¢s productivity as production workers are one of the key employee groups in the company. 3. 2 Problem #2 ââ¬â Performance Management System * Performance Appraisal system After conducting research interviews with production workers, it is understandable that the companyââ¬â¢s performance management system had many defects that reduce its effectiveness. Effectiveness of the existing performance management system in Cable Co is very doubtful for the reason listed below:Key performance criteria for both managerial and non-managerial employees are the same. Appraisal performance results have been kept confidential from the evaluated employees. Evaluations made upon appraiserââ¬â¢s daily observations and individual judgments, make th e current system very subjective and results do not have validity. The company currently doesnââ¬â¢t have job analysis for most of its positions, which makes it really hard to evaluate the employeesââ¬â¢ performance or to explain what are the main responsibilities and tasks for a position. Bonus System Currently there is no bonus system in the company. The previous system was used more to avoid potential conflict between employees and lost its function as a motivational tool. The bonus system based on defected performance evaluation process fails and decreases employees satisfaction. Operating in such a highly competitive market, it is essential to have motivated employees, and tools that help to keep them engaged. 3. 3 Problem #3 ââ¬â Recruitment & Selection * Applicants databaseBecause the company does not have a common computerized database for all applicants, the recruitment process for every new position is more costly and more time consuming. Previous unsuccessful ca ndidates and interview records might be useful for the next recruitment project, but it is difficult to find this information without a database. * Candidate selection There are no common guidelines for the department managers to use when conducting the recruitment process, no selection criteria that would be based on and aligned with the companyââ¬â¢s business strategy. 4.Proposed Solution To make changes in the organizationââ¬â¢s structures, systems or processes managers should have support from people working within the organization otherwise it will be impossible (Fombrun and Nevins, 2004, p. 209). It is suggested that inefficient Human Resources Management system in the company can be improved by more effective HR planning, a reviewed performance management system and recruitment and selection processes. 4. 1 Solution #1 ââ¬â Human Resources Planning 1. Organize meetings between Cable Co Board of directors, General Manager and Executive Manager.The objectives for this meeting should be: * To review companyââ¬â¢s goals and business strategy; * To identify and review all Cable Co target market segments; * To organized repeated market research to evaluate domestic (China) market demand for their product; * Based on research, reformulate the companyââ¬â¢s business development strategy; * Management team should always be present, especially if the company put such a great emphasis on management control in its business strategy and product quality. Formulate Human resources strategy aligned with business development goals that will enable to plan the companyââ¬â¢s people resources more efficiently. A previously mentioned problem is that the company is unable to offer accommodation for its employees should be seriously reconsidered. * Review employment offer package ââ¬â concentrating on accommodation facilities for employees other than those from the local area. * That way companyââ¬â¢s candidate pool would be extended, that can increa se the best applicant attraction and give the company a competitive advantage. Organize a team to conduct accommodation market research; * Revise the companyââ¬â¢s opportunities to offer accommodation or a housing allowance for their employees. * This will expand the companyââ¬â¢s candidate pool and stabilize the workforce and turnover. 4. 2. Solution #2 ââ¬â Performance Appraisal System ââ¬Å"Employee Performance Management is a process for establishing a shared workforce understanding about what is to be achieved at an organization level. It is about aligning the organizational objectives with the employees' agreed measures, skills, competency requirements, development plans and the delivery of results. (http://www. peoplestreme. com/what-is-performance-management. shtml, 20/05/2012) It is essential to make sure employees know what the positions key requirements are for high performance are. It is hard to maintain or try to achieve any performance level if the employee is not aware what the key aspects they have been evaluated for are. It is recommended to revise key performance criteria for managerial and non managerial positions in the company. These key areas for both types of employees should be formulated in the positions job description.When conducting job analysis, human resources personnel should consider the companyââ¬â¢s business strategy. In this case the company pursues cost leadership and differentiation strategies. This kind of business usually requires a workforce who are adaptable, efficiency oriented, trainable and willing to follow standardized procedures (Gully, M. , Phillips M. 2009). Firstly, it is suggested to determine key performance criteria for managers and all other employees, and show those in their job description. Job analysis, which include important aspects of a job and characteristics a worker needs to do it well (Gully, M. Phillips M. 2009) should be conducted for each position in the company. Job descriptions should be received by each employee on the day they sign the employment contract with the company. That way the employee clearly understands the companyââ¬â¢s requirements for high performance and key performance criteria for their positions. Secondly, a new performance appraisal form should be developed (Appendix 1 ââ¬â Performance Appraisal form), which allows matching the key performance criteria from the job description for different positions.An evaluation process should still be conducted from the top to the bottom, but it is important to have open communication and feedback on the results after employee evaluation. Employees wonââ¬â¢t improve if managers or supervisors wonââ¬â¢t give them honest and constructive feedback on their previous performance against the key criteria. As this is a new thing for supervisors and managers, it is recommended to set up guidelines on how to give constructive feedback about an employeeââ¬â¢s performance (Appendix 2 ââ¬â G iving feedback model). It is also advisable to process 360 degree feedback for companyââ¬â¢s managers and supervisors.This would give managerial team a better understanding of their strengths and weaknesses and an insight on how others perceive them. Also provide an opportunity to adjust behaviors and develop skills that will enable them to excel at their jobs (http://www. custominsight. com/360-degree-feedback/what-is-360-degree-feedback. asp, 12/05/2012). A bonus system should be developed for key areas of the company such as Marketing and also Production Departments. It is also recommended to create an incentive plan for all employees if the company has reached its goal at the end of the year (for example, specific amount of sales or profit).That way all employees would feel rewarded, because everyone has contributed to improve productivity, profitability and to keep the organizationsââ¬â¢ competitive advantage. Employee performance appraisal form results should also be tak en into consideration when building the bonus system. It should be based on sales results for the Marketing department and productivity and quality for the Production department employees. The company should consider a yearly bonus implementation for all employees if an annual target has been reached.A bonus system is a great motivational tool that means more motivated employees, better work engagement, improved productivity and product quality, which help the company to gain better profitability. 4. 3 Solution #3 ââ¬â Recruitment and Selection process * Candidate Database It is recommended to invest in the companyââ¬â¢s development by creating a computerized HRM system, which includes all previous applicants and records on current employees. Included information on TeleCo employees would be a great source for Cable Co further recruitment processes.In this HRM system the company could also create talent inventories ââ¬â detailed records that summarize each employeeââ¬â ¢s skills, competencies, and qualifications (Gully, M. , Phillips M. 2009, p. 126. ). This can be used as a very helpful tool in filling in future vacancies and also developing succession management in the company. * Recruitment and Selection If the company keeps involving line managers and supervisors in Human Resources processes, the selection and recruitment guidelines for management and supervisorââ¬â¢s team should be developed.It is important to have a formal instructions, and processes to follow when selecting, attracting, assessing and deploying employees (Gully, M. , Phillips M. 2009). Supervisors have to be competent to explain to the new employees what the key areas of the position are and what level of performance is expected from them. This information would be easily found in the job descriptions for each position, based on job analysis and performance appraisal reviews. These recommended changes will help Cable Coââ¬â¢s leadership team to improve the Human Resour ces management in the company and increase management control over organizationââ¬â¢s processes.Reference List Gully. M, Phillips M. (2009). Strategic Staffing (2nd ed. ) Upper Saddle River, New Jersey: Pearson Education Fombrun. J, Nevids D. (2004). The Advice Business. Essential Tools and Models for Management Consulting, Upper Saddle River, New Jersey: Pearson Education Warner J. (2002) The Janus Performance Managament System: A Complete Performance Management Support Process for Individuals, Teams and the Entire Organization (Volume I), HRD Press Zhu J. C. Organizations Development and Change. Changing HR practices in China ââ¬â Cable Co. Case Study A PeopleStream Pty. Ltd. Performance management. Retrieved from http://www. peoplestreme. com/what-is-performance-management. shtml Custom Insight. com. 360 Degree Solutions. Retrieved from http://www. custominsight. com/360-degree-feedback/what-is-360-degree-feedback. asp Business Balls, Performance Appraisal forms. Retrieved from http://www. businessballs. com/performanceappraisals. htm
Wednesday, January 8, 2020
Rational And Hypothesis Factors That Affect The Rate Of...
Rational and Hypothesis: My hypothesis in this aim is that mutations in UPF1 inhibit NMD thereby stabilizing premature transcripts that are translated to produce truncated proteins which promote pancreatic ASC. Previous studies have demonstrated that a subset of transcripts inhibited upon UPF1 knockdown are stabilized and translated. My objectives in this aim are: a) To determine global occupancy of mutant UPF1 on RNA b) To analyze if the mutant UPF1 bound RNA fragments are translated c) To determine which of the targets are essential to promote tumorigenesis. A. To identify direct targets of UPF1 mutants in pancreatic cancer. One challenge facing study of UPF1 and mammalian NMD is identification of direct regulatory targets. While genomeâ⬠¦show more contentâ⬠¦Briefly, cells will be treated with cyclohexamide to arrest translating ribosomes. Extracts from these cells will be treated with RNase I to degrade regions of mRNAs not protected by ribosomes. The resulting 80S monosomes, which contain a Ã¢Ë ¼30-nucleotide RPF, will be purified on sucrose gradients and then treated to release the RPFs, which are then processed for Illumina high-throughput sequencing. In parallel, poly (A)-selected mRNA from each sample was randomly fragmented, and the resulting mRNA fragments will be processed for sequencing (mRNA-Seq) using the same protocol as that used for the RPFs. In summary, these experiments will pinpoint the targets, which are translated in UPF1 mutant cells. C. CRISPR depletion screen to identify bonafide NMD targets In this sub aim, my goal is to identify the fraction of NMD targets that are essential for UPF1 mediated tumorigenesis. For this purpose, I will collaborate with Dr. Vidigal who has developed a rapid cloning method for generating double guide-paired library and a computational tool for selecting guides that have minimum off-targets. Based on results from Aim 1 and 2a, I will design a customized library targeting UPF1 bound RNA targets. I will infect the library at low multiplicity of infection (MOI) into HPNE KRAS UPF1 mutant cells. I will culture cells for 30 days post transduction to maximize the identification of UPF1 mutant targets
Tuesday, December 31, 2019
Essay On The Movie Thunderheart - 837 Words
The 1992 film, Thunderheart, directed by Michael Apted and written by John Fusco, portrays young FBI agent, Ray Levoi, who is a quarter Sioux Indian, investigating a homicide case on a Native American reserve. The movie incorporates themes of self-acceptance and discovery as Levoi reflects on who he is and discovers not only himself and his culture but the ugly truth about the reserve. There is a scene where Maggie questions Ray on whether he is Indian or not, he only agrees because wants to speak with the old woman, but you can visually see the disgust as he says yes. This is the same disgust he had when Coutelle mentioned that they were his people. Which is ironic, because at the end of the movie when Crow Horse refers to theâ⬠¦show more contentâ⬠¦Maggie strives to be improve her community and assist her people. She has devoted her life to their rehabilitation by earning a degree from Dartmouth to be able to organize local control of the schools and form a womens shelter. After this inspiration had shocked Ray, he comes to yearn for the same connection she has with her people as he never got chance for isolating that part of himself. Thunderheart incorporates much of the Native American culture from the nicknames to the powwow. Ray is referred to as many things including Sal, meaning fake little Indian and kola, meaning good friend. (Carly 9) A powwow is where Ray followed Crow Horse and Reaches to; it is a ceremonial gathering involving singing, dancing, and feasting. The introduction to the movie accurately depicts the Indians performing the Ghost Dance which was said to be done when the messiah has come down to Earth to salvage the Indians. (Weiser 2) Symbolism is a key feature in Thunderheart as it is for the Native Americans. When asked about Leos murder Reaches mentions that he sees an owl, a symbol that someone will die. Ray sees that same owl before he finds Maggies body in the mining field. Ray is told by both Crow Horse and Reaches to list en to the water, which we later find out that the water has become contaminated because of the mining fields. This is an example of how the indigenousShow MoreRelatedThunderheart Analysis1093 Words à |à 5 PagesNative Americans from their lands and placed them on reservations where they can hardly carry out any of their customs. The motion picture, Thunderheart, tells an adaptation of the incident at Oglala, where the main protagonist, FBI agent Raymond Levoi, and his partner Frank Coutelle have to investigate the murder of a local pro-government Indian supporter. Thunderheart conveys three main images throughout the film which includes ceremonial tobacco pipe to show that American government agencies alwaysRead MoreWhy I Am A Writer Essay1633 Words à |à 7 Pagespaper. From the day I got my paper back I did not stop revising it. I had revised this paper almost three times, and I will still be revising it till the final portfolio is due. This comes to show that there is no such thing as a perfect paper. Every essay that I write can always be improved. This paper has taught me a lot about truly learning from my slipups and avoiding on doing it again in the future. The Murray exercise for this paper helped me. As important as the creative voice that helpsRead MoreMy Journey As A Writer Essay2447 Words à |à 10 Pagesrealized the importance of knowing your subject before you write about them. Before, I thought as long as I know even just a tad bit of information, I can write a five paragraph essay. But, through this material I realize that this was a mistake and now I make sure that I know enough information for me to construct a better essay. I expanded my knowledge of Native Americans through reading , I Tell You Now. At first, I did not understand the purpose of taking notes while reading. I thought that
Sunday, December 22, 2019
The Future Of Currency Essay - 3241 Words
The Future of Currency nbsp;nbsp;nbsp;nbsp;nbsp;In the present day, the worlds economy is ever-changing and adjusting. Many different reasons control the reasons for this. The future of currency is something that can only be predicted and is not guaranteed. However, there are many determing factors behind the changes that can take place. Asia and North America are two continents that have economies that have recently changed or are in the midst of change. World War 2 drew a hard blow and left a serious and lasting effect to many Asian countries. This however, did not hamper the growth of countries such as China, Japan and Vietnam as their governments were taking serious steps to recover economically. Thus, the global market cannotâ⬠¦show more contentâ⬠¦In accordance to this, China went on a construction binge. Whole factories were purchased from abroad while others were built with local resources. By 1978, the frenzy for new projects reached a level that reminded some people of the Great Leap Forward. In an effort to promote agricultural production, the government released many of the restrictions on the spontaneous capitalist tendencies of the peasantry. (173) In the late 1980s, the government decided to expand the scope of private marketing. The next step was to increase the amount land assigned to the peasants. The peasants were now not responsible to the government for the use they made to the private plots. They simply co uld grow what they wished, for the sale to the government or to private markets. This led to furious rebuilding and inflow of foreign investments. All this enabled China to remake itself into Asians hub of finance, trade and culture. By 1984, they were producing more than $1 million worth of rice and a range of side products, including rice wine. Their residential earning was up to about $200 a year. (Prager 52 ) This meant that they could begin replacing their mud-and-straw hats with solid brick houses. Shanghai today is a vast construction site with more than 20,000 projects, with 27,000 companies building bridges, tunnels, flyovers, ring roads, hotels, villas, golf courses and also public housing. This sparked national growth of about 10% a year.( 53 ) The Chinese now are going homeShow MoreRelatedNotes Currency And Its Future2165 Words à |à 9 PagesPaper Currency and Its Future Currency has long been used as a tool to purchase things like goods and services. The many transactions of these early times involved the exchange of both coins and paper. However, in our modern era of cell phones and plastics, these primitive methods of payment may be coming to an end. With the introduction of credit cards and easy payment via cellular, this outcome may not be very far off. 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The unregulated version of capitalism employed and protected by the United States Government (USG) was developed by individual entrepreneurs operating in the freest market in the world. They innovated petroleum refining and business practices that are still supporting U.S. global monetary control and financial power today. Now that humanity is making a shift away fromRead MoreMultinational Financial Management by Alan Shapiro: Currency Futures and Options Markets1250 Words à |à 5 Pagesï » ¿CHAPTER 8 Currency Futures and Options Markets EASY (definitional) 8.1 Which one of the following currency futures contracts is currently NOT available? a) French franc b) Hungarian forint c) Czech koruna d) Norwegian krone Ans: a Section: Futures contracts Level: Easy 8.2 Which of the following has provided a major inducement for speculators to participate in the futures market? a) low margin requirements b) low bidââ¬âask spreads c) high volume compared to the forward market d)Read MoreTypes Of Foreign Exchange Risks981 Words à |à 4 Pages Financial hedging is the use of hedging instruments - typically FX forwards, options and swaps - that are sold by foreign exchange brokers and banks to reduce company exposure to currency fluctuations. (Export Development Canada, 2016) Acknowledging that foreign exchange risk is a real threat to corporate cash flows, assets and expenses can be one of the biggest hurdles for most companies. However, recognizing the importance of protecting assets companies can move forward in identifying the typeRead MoreCurrency Derivatives1372 Words à |à 6 PagesCurrency derivatives Introduction Currency derivatives come in to existences as a hedging tool. As against unfavourable appreciation and depreciation of a single currency. Exporter, importer and financial investor have developed a vast range of currency derivative instruments are also used by speculators willing to arrange future currency selling or buying contracts while hoping hoping to buy or sell the currency at favourable anticipated exchange rates in the future. This act of speculatorRead MoreCurrency Hedging970 Words à |à 4 Pagesposed by worldwide currency fluctuations. One hedges the currency risk by contracting to sell foreign currency in the future, at the current exchange rate (Fries). If fund managers think the dollar is going to be stronger when they are ready to change the foreign currency back into American dollars, then they take out a foreign futures contract (a hedge). Thus, they lock in the exchange rate beforehand, so that they will not lose profits gained from holding devalued foreign currenc y (Hedging, 1999)
Saturday, December 14, 2019
All About Me . Free Essays
Shadira Andrews Writing #5 Night My whole heart stopped when I saw the blood dripping from my arm. Today was a regular day but my whole life changed. It was a sunny day with my cousins, sister, friends and I when suddenly I get a huge scar on my right back-hand. We will write a custom essay sample on All About Me . or any similar topic only for you Order Now It might look like a burn but itââ¬â¢s not, itââ¬â¢s a terrible long lasting scar. This raggedy old scar is about nine years old but I still remember every single detail of how this happened. An almost trip to the hospital wasnââ¬â¢t supposed to happen with just a game of basketball that I wasnââ¬â¢t playing. This almost fun day was one of the worst days of my life; this was the night of my life. Why do we have to play tag? This game makes me very exhausted but I have to other wise my crush is going to think I am fat. I am going to need about five bottles of Gatorade to stay energized though. We played tag around the school-yard for about 15 minutes but I had to use the restroom so I told everyone that Iââ¬â¢ll be back in a couple of minutes. Just before I was completely out of the yard my sister called my name to ask me to get her something from the house. The next thing you know a boy ends up accidently knocking me unto the floor and I slide across the concrete. If I was car and I was making a sharp turn you would only hear me screaking. The boy was playing basketball and I happened to stop right in front of the basketball court. I canââ¬â¢t believe Iââ¬â¢m lying on this concrete with nothing but blood and a boy I donââ¬â¢t even know next to me. I blacked out for a moment and couldnââ¬â¢t hear anything, it was so silent but everyone talked. All I could see and remember is the faces on everyoneââ¬â¢s face. My sister looked as if she was having a baby, and the boy looked as if he saw something ghastly. Everyone was panicking but what I couldnââ¬â¢t get out of my head is the fact that my stockings ripped; those were new stockings that my mom finally got me so you could say my facial expression was being annoyed and irritated. By now I have snapped out of my black out. My cousins helped me up while the shamefaced boy offered me bandages. I just remember I really have a full bladder right about now. Night, pain, dusk, and agony is all that filtered the air as I was being carried by a dozen of my relatives, I mean come on was I that heavy did my crush need to know I needed half of dozen people carrying me. All I was thinking was ââ¬Å"Am I going to get into trouble for ripping my brand new stockings? â⬠As soon as I got into the house my dad asked what happened and I just said ââ¬Å"I fell. â⬠I didnââ¬â¢t want my chaos to turn into a royal rumble or something. My dad did the worst thing ever! He grabs alcohol, peroxide, and everything else thatââ¬â¢s expected to stings. I automatically start to cry knowing that the felling will soon be excruciating pain. Of course I hate pain and honesty I didnââ¬â¢t feel anything when my skin came off my right back-hand; but when my dad put that alcohol on my open wound I could have said every curse word in the book. Right about now I was speaking gibberish, the pain was unbearable and I couldnââ¬â¢t understand why my dad didnââ¬â¢t just take me to the hospital he is not a doctor. I guess you could say a dad is anything and everything it needs to be at the time being. Pain is such an uncomfortable feeling that even a tiny amount of it is enough to ruin a week. My hand was throbbing and I couldnââ¬â¢t move it or think about it because that just made the situation worst. I tried a lot of things to make the pain fade away. I tried eating ice cream, going to sleep, and even putting a cold rag around the bandage nothing worked. Then, everyone decided to leave me in the house by myself. My mom and dad had to work and my siblings just took it upon themselves to go outside and not tell me. I looked at the clock and itââ¬â¢s approximately five minutes to 12 when there is a knock on the door. Now I am not going to lie I was little scared to answer the door since it was so late so since I was short I climbed on the couchââ¬â¢s leg and looked throw the peep hole. It was my crush! Was he going to make fun of me, letââ¬â¢s see? Everything I did our little conversation was the only thing that helped me block out the pain, of course I was blushing he was adorable. I was finally coming into the house with a smile on my face today and that smile easily went away when I saw the time on my round clock, it said it was 12:11 A. M. In conclusion, today was the worst day ever I got hurt pretty bad with a scar that will be on my back-hand for life and I for the first time ever I now know what pain really feels like. I also have a fear of basketball now no lie; every time I play basketball I always seem to get hurt either I get hit in the head with the ball or I hurt my fingers and they become swollen. Every time someone ask me what happened to my hand and if I were burnt, I say no and I have to summarize this whole story. I try not to remember and forget but I canââ¬â¢t forget that pain. ââ¬Å"Pain has an element of blankâ⬠Emily Dickinson. How to cite All About Me ., Essay examples
Friday, December 6, 2019
Leadership Style
Question: Discuss about the Leadership style Determined Situation ? Answer: Introduction The leadership style is determined as per the specific situation. There are some situations in which the leader manages the crisis without consulting the followers. Thus leadership style is determined as per the demand of the situation (Bradberry, 2015). There are different leadership styles which are commonly followed by the leaders. These leadership styles are such as: autocratic, democratic, transformational, transactional, laissez-faire and charismatic. All these styles are applied by the leaders as per the need of the situations. Characteristics of the leadership styles Each category of leadership style possesses different approaches which are required to be followed by the leaders to manage the situation and to lead the people. Autocratic leadership style In this form of the leadership style the leaders keeps the authority responsibility in his or hands. The leader makes decision without taking any help from the followers. The leader communicates the decisions to the employees and expects the subordinate to implement the decisions quickly. There is no flexibility in this kind of leadership style (Kannair, 2007) Democratic leadership style The democratic leadership style focuses on involving the followers in the process of the decision making. The leader remains in the center of all activities and his or her followers contribute to the leader. The final responsibility remains with the leader but the leader delegates authority to the followers to take decision in the progress of the workplace culture. In this style of leadership, the culture of fairness, competence, creativity, honesty and intelligence are found. Transformational leadership style It is one of the most important leadership styles in the modern organisation. The leadership style focuses on making initiation for the changes in the organisation and in groups. The transformational leader is popular for motivating people to contribute more in comparison to the intended output (Walton, 2008). The higher performance is achieved by setting more challenging expectations. Laissez-faire leadership style It gives the employees authority and they are allowed to work without being interfered. The leadership style is found in the situation where the leader gets least satisfaction. The subordinates get more advantage to take decisions for their own jobs. Transactional leadership style The transactional leadership style focuses on achieving the goals in the process of the exchange. The followers are awarded for executing the vision of the leader. The followers get rewards and in the opposite situations they followers are given punishment for doing their job. The leadership style focuses more on the need of clarifying the expectation of the performance of the followers. It also explains how to achieve the expectations (Sandel, 2009). The style allocates rewards that meet the objective of the organisation. Charismatic leadership style The charismatic leadership style refers to the ability of the leader to make a transfer of the values beliefs of the followers. To become a charismatic, one requires having a structure of specific attitudes. Apart from the above six important leadership styles a leader requires to be a visionary. The leader requires possessing outstanding ability to transform the vision into reality. The modern organisations are based on corporate and global culture. The employees of the organisation are from the different cultures. The leader requires dealing the employees from the different culture and focusing more on understanding people and their expectations (Greyvenstein Cilliers, 2012). This will help the leaders to lead the people in the right direction. Motivating people to accept the change and the culture of the organisation is one of the important styles of the leaders. The leader requires motivating people by fulfilling their expectations and supporting them to excel both in their social and professional life. Conclusion The leader exhibits different styles as per the need of the situations. A true leader understands the situation and utilizes the knowledge and intelligence as per the demand of the situation. The success of the organisation depends on how the leader leads the people in different situations. The different leadership styles educate the leaders to be effective in managing the situations. Referances Bradberry, T. (2015, 07 16). Are You a Leader or a Follower? Retrieved 04 18, 2017, from entrepreneur.com: https://www.entrepreneur.com/article/248293 George-Godfrey, E. S. (2012, 09 13). The Role Of Follower in Leadership. Retrieved from kaizenbiz.com: https://kaizenbiz.com/the-role-of-follower-in-leadership/ Greyvenstein, H., Cilliers, F. (2012). Followership's experiences of organisational leadership: A systems psychodynamic perspective. South African Journal of Industrial Psychology, 38(2), 1-10. Kannair, J. (2007). The Ethical Mind. Harvard Business Review, 85(3), 51-56. Sandel, M. J. (2009). Justrice: What's the right thing to do? New York: Farra, Straus and Giroux. Walton, B. (2008). The ethical executive. Library Journal, 133(19), 79-80.
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